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Once the semen enters the ductus epididymis the principal cells, which contain pinocytotic vessels indicating fluid reabsorption, secrete glycerophosphocholine which most likely inhibits premature capacitation.

The accessory genital ducts, the seminal vesicle, prostate glands, and the bulbourethral glands, produce most of the seminal fluid.

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These alkaline bases counteract and buffer the acidic environment of the vaginal canal, and protect DNA inside the sperm from acidic denaturation.The volume of semen ejaculate varies but is generally about 1 teaspoonful or less.A review of 30 studies concluded that the average was around 3.4 milliliters (ml), with some studies finding amounts as high as 5.0 ml or as low as 2.3 ml.The process that results in the discharge of semen is called ejaculation. In animals, semen has been collected for cryoconservation.Cryoconservation of animal genetic resources is a practice that calls for the collection of genetic material in efforts for conservation of a particular breed.

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